Filarial selenium glutathione peroxidase: a probable immunodiagnostic marker for lymphatic filariasis.
Identifieur interne : 005893 ( Main/Exploration ); précédent : 005892; suivant : 005894Filarial selenium glutathione peroxidase: a probable immunodiagnostic marker for lymphatic filariasis.
Auteurs : Anchal Singh [Inde] ; Shaukat Kamal ; Sushma RathaurSource :
- Transactions of the Royal Society of Tropical Medicine and Hygiene [ 1878-3503 ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux, Antigènes d'helminthe (immunologie), Antigènes d'helminthe (isolement et purification), Antigènes d'helminthe (sang), Bovins, Filariose lymphatique (diagnostic), Filariose lymphatique (immunologie), Glutathione peroxidase (immunologie), Humains, Immunoglobuline G (immunologie), Marqueurs biologiques (sang), Reproductibilité des résultats, Sensibilité et spécificité, Sélénium (immunologie), Test ELISA.
- MESH :
- diagnostic : Filariose lymphatique.
- immunologie : Antigènes d'helminthe, Filariose lymphatique, Glutathione peroxidase, Immunoglobuline G, Sélénium.
- isolement et purification : Antigènes d'helminthe.
- sang : Antigènes d'helminthe, Marqueurs biologiques.
- Animaux, Bovins, Humains, Reproductibilité des résultats, Sensibilité et spécificité, Test ELISA.
English descriptors
- KwdEn :
- Animals, Antigens, Helminth (blood), Antigens, Helminth (immunology), Antigens, Helminth (isolation & purification), Biomarkers (blood), Cattle, Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (immunology), Enzyme-Linked Immunosorbent Assay, Glutathione Peroxidase (immunology), Humans, Immunoglobulin G (immunology), Reproducibility of Results, Selenium (immunology), Sensitivity and Specificity.
- MESH :
- chemical , blood : Antigens, Helminth, Biomarkers.
- chemical , immunology : Antigens, Helminth, Glutathione Peroxidase, Immunoglobulin G, Selenium.
- chemical , isolation & purification : Antigens, Helminth.
- diagnosis : Elephantiasis, Filarial.
- immunology : Elephantiasis, Filarial.
- Animals, Cattle, Enzyme-Linked Immunosorbent Assay, Humans, Reproducibility of Results, Sensitivity and Specificity.
Abstract
Lymphatic filariasis (LF) caused by Wuchereria bancrofti is widely prevalent in tropical and subtropical countries. Night blood film examination is most commonly used for diagnosis of filariasis but is cumbersome and labour intensive. In order to develop an indirect ELISA-based immunodiagnostic test, the importance of antifilarial IgG subclasses was evaluated in bancroftian filariasis patients. Blood samples from healthy individuals and different categories of LF patients were used to estimate the diagnostic potential of selenium glutathione peroxidase antigen purified from the bovine filarial parasite Setaria cervi. This antigen reacted with both IgG(1) and IgG(4); however, the IgG1 response was greater in microfilaraemic patients and the IgG(4) response was higher in chronic filarial patients. The diagnostic sensitivity of IgG(1) and IgG(4) was 97% and 96% whereas specificity was determined to be 95% and 98% respectively. Our observations suggest that SeGSHPx could be an alternative diagnostic marker for the detection of bancroftian filariasis in an endemic area.
DOI: 10.1016/j.trstmh.2010.02.007
PubMed: 20227740
Affiliations:
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last="Rathaur">Sushma Rathaur</name></author></analytic><series><title level="j">Transactions of the Royal Society of Tropical Medicine and Hygiene</title><idno type="eISSN">1878-3503</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antigens, Helminth (blood)</term><term>Antigens, Helminth (immunology)</term><term>Antigens, Helminth (isolation & purification)</term><term>Biomarkers (blood)</term><term>Cattle</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (immunology)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Glutathione Peroxidase (immunology)</term><term>Humans</term><term>Immunoglobulin G (immunology)</term><term>Reproducibility of Results</term><term>Selenium (immunology)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" 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qualifier="isolation & purification" xml:lang="en"><term>Antigens, Helminth</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Filariose lymphatique</term><term>Glutathione peroxidase</term><term>Immunoglobuline G</term><term>Sélénium</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antigènes d'helminthe</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Marqueurs biologiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Bovins</term><term>Humains</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lymphatic filariasis (LF) caused by Wuchereria bancrofti is widely prevalent in tropical and subtropical countries. Night blood film examination is most commonly used for diagnosis of filariasis but is cumbersome and labour intensive. In order to develop an indirect ELISA-based immunodiagnostic test, the importance of antifilarial IgG subclasses was evaluated in bancroftian filariasis patients. Blood samples from healthy individuals and different categories of LF patients were used to estimate the diagnostic potential of selenium glutathione peroxidase antigen purified from the bovine filarial parasite Setaria cervi. This antigen reacted with both IgG(1) and IgG(4); however, the IgG1 response was greater in microfilaraemic patients and the IgG(4) response was higher in chronic filarial patients. The diagnostic sensitivity of IgG(1) and IgG(4) was 97% and 96% whereas specificity was determined to be 95% and 98% respectively. Our observations suggest that SeGSHPx could be an alternative diagnostic marker for the detection of bancroftian filariasis in an endemic area.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Kamal, Shaukat" sort="Kamal, Shaukat" uniqKey="Kamal S" first="Shaukat" last="Kamal">Shaukat Kamal</name><name sortKey="Rathaur, Sushma" sort="Rathaur, Sushma" uniqKey="Rathaur S" first="Sushma" last="Rathaur">Sushma Rathaur</name></noCountry><country name="Inde"><noRegion><name sortKey="Singh, Anchal" sort="Singh, Anchal" uniqKey="Singh A" first="Anchal" last="Singh">Anchal Singh</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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